pH-Dependent Conformational Change of Gastric Mucin Leads to Sol-Gel Transition
| Autor: | Xingxiang Cao, K. Ramakrishnan Bhaskar, Rama Bansil, J. Thomas Lamont, Nezam H. Afdhal, Bradley S. Turner, Niu Niu |
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| Rok vydání: | 1999 |
| Předmět: |
Models
Molecular Conformational change Light Macromolecular Substances Protein Conformation Swine Diffusion Static Electricity Biophysics Biophysical Phenomena Hydrophobic effect Protein structure Dynamic light scattering Static electricity Animals Scattering Radiation Fluorescent Dyes Binding Sites Chemistry Gastric Mucins Mucin Mucins Gallbladder Hydrogen-Ion Concentration Random coil Solutions Crystallography Cattle Gels Research Article |
| Zdroj: | Biophysical Journal. 76:1250-1258 |
| ISSN: | 0006-3495 |
| Popis: | We present dynamic light scattering (DLS) and hydrophobic dye-binding data in an effort to elucidate a molecular mechanism for the ability of gastric mucin to form a gel at low pH, which is crucial to the barrier function of gastric mucus. DLS measurements of dilute mucin solutions were not indicative of intermolecular association, yet there was a steady fall in the measured diffusion coefficient with decreasing pH, suggesting an apparent increase in size. Taken together with the observed rise in depolarized scattering ratio with decreasing pH, these results suggest that gastric mucin undergoes a conformational change from a random coil at pH≥4 to an anisotropic, extended conformation at pH |
| Databáze: | OpenAIRE |
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