TWEAK /Fn14 mediates atrial‐derived HL ‐1 myocytes hypertrophy via JAK 2/ STAT 3 signalling pathway
Autor: | Xin Yue, Manyi Ren, Bing Rong, Yachao Zhao, Mingjie Lin, Fei Xie, Li Hao, Wenqiang Han, Jing-Quan Zhong |
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Rok vydání: | 2018 |
Předmět: |
Male
0301 basic medicine Cardiomyopathy Muscle hypertrophy Mice TWEAK Atrial Fibrillation Myocyte Myocytes Cardiac RNA Small Interfering STAT3 biology Cytokine TWEAK Atrial fibrillation Middle Aged Hedgehog signaling pathway TWEAK Receptor cardiovascular system Molecular Medicine Female Original Article hypertrophy Atrial Natriuretic Factor Signal Transduction STAT3 Transcription Factor medicine.medical_specialty Primary Cell Culture Atrial Appendage Cardiomegaly 03 medical and health sciences Troponin T Internal medicine medicine Animals Humans Heart Atria cardiovascular diseases Aged business.industry JAK2/STAT3 Fn14 Original Articles HL‐1 atrial myocytes Cell Biology Janus Kinase 2 medicine.disease Troponin Disease Models Animal 030104 developmental biology Endocrinology Gene Expression Regulation Case-Control Studies Leukocytes Mononuclear biology.protein business |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | Atrial myocyte hypertrophy is one of the most important substrates in the development of atrial fibrillation (AF). The TWEAK/Fn14 axis is a positive regulator of cardiac hypertrophy in cardiomyopathy. This study therefore investigated the effects of Fn14 on atrial hypertrophy and underlying cellular mechanisms using HL‐1 atrial myocytes. In patients with AF, Fn14 protein levels were higher in atrial myocytes from atrial appendages, and expression of TWEAK was increased in peripheral blood mononuclear cells, while TWEAK serum levels were decreased. In vitro, Fn14 expression was up‐regulated in response to TWEAK treatment in HL‐1 atrial myocytes. TWEAK increased the expression of ANP and Troponin T, and Fn14 knockdown counteracted the effect. Inhibition of JAK2, STAT3 by specific siRNA attenuated TWEAK‐induced HL‐1 atrial myocytes hypertrophy. In conclusion, TWEAK/Fn14 axis mediates HL‐1 atrial myocytes hypertrophy partly through activation of the JAK2/STAT3 pathway. |
Databáze: | OpenAIRE |
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