Separation of amino acid phenylthiohydantoin derivatives by high-pressure liquid chromatography
| Autor: | Joseph L. Meuth, J. Lawrence Fox |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
Chromatography Aqueous normal-phase chromatography Edman degradation Chemistry Hydantoins Microchemistry Hydrophilic interaction chromatography Biophysics Cell Biology Hydrogen-Ion Concentration Biochemistry High-performance liquid chromatography Phenylthiohydantoin Amino acid Residue (chemistry) Protein sequencing Organic chemistry Amino Acid Sequence Amino Acids Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Analytical Biochemistry. 154:478-484 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(86)90018-7 |
| Popis: | Separation of the phenylthiohydantoin (PTH) derivatives of all 20 common amino acids is accomplished in approximately 11 min with excellent resolution by using high-pressure liquid chromatography. The chromatography is achieved at 50 degrees C on an Altex reversed-phase PTH-C18 column in an ammonium acetate-buffered acetonitrile, pH 4.5, mobile phase. Simple isocratic and linear gradient steps are used. Retention times for the various PTH-amino acids are very reproducible. Because the baseline is flat and free of background noise, PTH-amino acids can be detected in the low picomole range. The simplicity of this chromatographic system allows it to be easily automated. |
| Databáze: | OpenAIRE |
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