Development and evaluation of two TaqMan assays for generic detection of Dickeya species
| Autor: | C. Zijlstra, Eisse G. de Haan, Sven Warris, Lia Groenenboom – De Haas, Jan M. van der Wolf, M.C. Krijger, E.C.P. Verstappen |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Blackleg Dickeya Plant Science Horticulture 01 natural sciences Refused sample material Biointeractions and Plant Health BIOS Applied Bioinformatics 03 medical and health sciences chemistry.chemical_compound Sensitivity TaqMan Diagnostics Accuracy biology fungi food and beverages biology.organism_classification Seed potato 030104 developmental biology chemistry Biochemistry Specificity Agronomy and Crop Science Seed testing DNA 010606 plant biology & botany |
| Zdroj: | European Journal of Plant Pathology 156 (2020) European Journal of Plant Pathology, 156, 311-316 |
| ISSN: | 1573-8469 0929-1873 |
| DOI: | 10.1007/s10658-019-01876-y |
| Popis: | Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs. |
| Databáze: | OpenAIRE |
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