PAX6 Dosage Effects on Corneal Development, Growth, and Wound Healing
| Autor: | Romana Kucerova, Jingxing Ou, John D. West, Ida Parisi, J. Martin Collinson, Natalie Dora |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
PAX6 Transcription Factor
Cellular differentiation Gene Dosage gene dosage wound healing Apoptosis Mice Transgenic Biology anterior segment Article Epithelium Andrology Cornea Mice cornea Cyclin E Lens Crystalline medicine Animals Paired Box Transcription Factors Eye Proteins Corneal epithelium Cell Proliferation Homeodomain Proteins Wound Healing Cell growth Cell Cycle Cell Differentiation Phosphoproteins Molecular biology eye diseases Pax6 Microcornea Repressor Proteins medicine.anatomical_structure Phenotype Gene Expression Regulation Eye development Mice Inbred CBA Trans-Activators PAX6 sense organs Wound healing Developmental Biology |
| Zdroj: | Dora, N, Ou, J X, Kucerova, R, Parisi, I, West, J D & Collinson, J M 2008, ' PAX6 dosage effects on comeal development, growth, and wound healing ', Developmental Dynamics, vol. 237, no. 5, pp. 1295-1306 . https://doi.org/10.1002/dvdy.21528 |
| DOI: | 10.1002/dvdy.21528 |
| Popis: | The requirement for correct dosage of the transcription factor Pax6 during corneal growth and development was investigated using the Pax6-overexpressing (PAX77) transgenic mouse. Transgenics had a microcornea phenotype due to failure of postnatal growth, associated with reduction in the number of cells layers in the corneal epithelium. Cell cycle progression was monitored using bromodeoxyuridine, p63, cyclin E, and phosphohistone-3 labeling: proliferation rates were higher in PAX77+ than wild-type, without a concomitant increase in apoptosis. Hence, failure of proliferation did not underlie microcornea. PAX77+ corneal epithelia had reduced levels of cytokeratin-12, and exhibited severe wound healing delay that, in contrast to Pax6+/- mice, could not be modulated by exogenous growth factors. PAX77+ lenses showed partial failure of lens fiber differentiation. The data demonstrate that anterior eye development is very sensitive to Pax6 dosage. Although there are similarities between the eye phenotype of Pax6 heterozygotes and overexpressing mice, there are also striking differences. Developmental |
| Databáze: | OpenAIRE |
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