The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: An in vivo study
| Autor: | Yajun Tang, Chongyun Bao, Zhanhong Liu, Jing Wang, Yang Xu, Hang Wang, Xingdong Zhang, Xiangdong Zhu, Yongli Gao, Rui Zhao, Hai Lin, Peijie Tan, He Qiu |
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| Rok vydání: | 2022 |
| Předmět: |
Type III collagen
QH301-705.5 Photoaging Biomedical Engineering Pharmacology Recombinant collagen Safety and efficacy Biomaterials Extracellular matrix chemistry.chemical_compound Dermis In vivo Hyaluronic acid medicine Biology (General) Biological effect Materials of engineering and construction. Mechanics of materials Skin repair Epidermis (botany) Chemistry Regeneration (biology) medicine.disease Skin regeneration medicine.anatomical_structure TA401-492 Biotechnology |
| Zdroj: | Bioactive Materials, Vol 11, Iss, Pp 154-165 (2022) |
| ISSN: | 2452-199X |
| Popis: | The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field, such as collagen or hyaluronic acid, etc. However, the biosafety and efficacy of these implants are still challenged. In this study, a skin damage animal model was prepared by UV-photoaging and recombinant humanized type III collagen (rhCol III) was applied as a bioactive material to implant in vivo to study its biological effect, comparing with saline and uncrosslinked hyaluronic acid (HA). Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment. Histological observation, specific gene expression and other molecular biological methods were applied by the end of the animal experiment. The results indicated that rhCol III could alleviate the skin photoaging caused by UV radiation, including reduce the thickening of epidermis and dermis, increase the secretion of Collagen I (Col I) and Collagen III (Col III) and remodel of extracellular matrix (ECM). Although the cell-material interaction and mechanism need more investigation, the effect of rhCol III on damaged skin was discussed from influence on cells, reconstruction of ECM, and stimulus of small biological molecules based on current results. In conclusion, our findings provided rigorous biosafety information of rhCol III and approved its potential in skin repair and regeneration. Although enormous efforts still need to be made to achieve successful translation from bench to clinic, the recombinant humanized collagen showed superiorities from both safety and efficacy aspects. |
| Databáze: | OpenAIRE |
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