Correlation Between Viral Load of HBV in Chronic Hepatitis B Patients and Precore and Basal Core Promoter Mutations
| Autor: | Soad Ghabeshi, Seyed Masoud Hosseini, Zohreh Sharifi, Mahmood Mahmoodian Shooshtari |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Hepatitis Monthly |
| ISSN: | 1735-3408 1735-143X |
| DOI: | 10.5812/hepatmon.7415 |
| Popis: | Background: More than two billion people have been exposed to hepatitis B virus (HBV) worldwide. Furthermore, four hundred million of them are infected with chronic HBV infection. The predominant mutation of the precore region involves a G to A change at nucleotide1896, which creates a premature stop codon at codon 28. Two mutations of A1762T and G1764A are reported as the most prevalent mutations in the basal core promoter (BCP). Objectives: The purpose of this study was to investigate the relationship between mutations in precore (PC) and basal core promoter regions, and the viral load. Patients and Methods: Fifty serum samples from patients with hepatitis B were used. Levels of liver enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at the same time of serological markers of hepatitis B by ELISA. HBV-DNA was extracted from the sera, and then PCR performed on the HBV-DNA extracted with the use of specific primer of gene C. HBV viral load was determined by real-time PCR. The PC/ BCP mutations were determined by applying Line Probe Assay technique. The data were analyzed using SPSS software, version 20. Results: Only 82% of the patients were HBeAb positive and 76% of the patients had basal core/ precore mutations and mean viral load was 3/7 × 106 ± 9/7 × 105 IU/ml. Prevalence of mutations in the precore and basal core promoter regions were 46% and 30%, respectively. Conclusions: Our data indicated that there is a statistically significant relationship between HBV viral load and mutations in precore region (P < 0.05). |
| Databáze: | OpenAIRE |
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