In vitro blood compatibility evaluation method: incubating while rotating hemodialyzers filled with fresh human blood
| Autor: | Toshinori Koizumi, Sayuri Tanba, Yuki Kishikawa, Satoru Inoue, Hiromi Tanaka, Kinue Kamata, Hatanaka Yoshihiro, Tokimizu Yusuke |
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| Rok vydání: | 2020 |
| Předmět: |
Blood compatible
030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) 030204 cardiovascular system & hematology Brief Communication Antioxidants Biomaterials Blood cell 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Renal Dialysis Antithrombotic property Anti-inflammatory property Evaluation methods medicine Humans Vitamin E Blood compatibility Polysulfone Human blood Membranes Artificial Vitamin E-bonded polysulfone membrane In vitro medicine.anatomical_structure Membrane chemistry Antioxidative property Cardiology and Cardiovascular Medicine Kidneys Artificial Biomedical engineering |
| Zdroj: | Journal of Artificial Organs |
| ISSN: | 1619-0904 1434-7229 |
| DOI: | 10.1007/s10047-020-01224-6 |
| Popis: | One of the often-used methods for in vitro evaluation of the blood compatibility of hemodialysis membranes is the circulation of human blood through a miniaturized hemodialyzer. The use of a rather small amount of human blood in its evaluation is one advantage of this method. However, because it is manufactured by a different process than actual ones, a miniaturized hemodialyzer membrane cannot always preserve the properties of actual hemodialyzers. To address this problem, we established a new experimental method that uses a relatively small amount of human blood and actual dialyzers. In this method, a test hemodialyzer and a control hemodialyzer filled with human blood obtained from the same donor is slowly rotated to prevent spontaneous blood cell sedimentation for 4 h at 37 °C. By use of this method, we were able to compare blood compatibility between a polysulfone (PS) membrane and a vitamin E (VE)-bonded PS membrane in terms of their relative antithrombotic, antioxidative, and anti-inflammatory properties. Consistent with many previous reports, the results clearly showed that compared with the PS membrane, VE-bonded PS membrane is more blood compatible. These findings suggest that our method is applicable, at least to in vitro blood compatibility evaluation of PS type dialysis membranes. |
| Databáze: | OpenAIRE |
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