Inhibition of swim bladder inflation in Japanese medaka (Oryzias latipes) embryos following exposure to select pharmaceuticals alone and in combination
| Autor: | John Guchardi, Zacharias Pandelides, M.D. Overturf, Douglas A. Holdway, Erin Ussery |
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| Rok vydání: | 2021 |
| Předmět: |
Diclofenac
Embryo Nonmammalian Health Toxicology and Mutagenesis Oryzias Urinary Bladder 010501 environmental sciences Aquatic Science 01 natural sciences Andrology 03 medical and health sciences Human fertilization Swim bladder medicine Animals Levonorgestrel Swim bladder inflation 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Estradiol biology Anti-Inflammatory Agents Non-Steroidal Embryo Japanese Medaka biology.organism_classification Water Pollutants Chemical medicine.drug Hormone |
| Zdroj: | Aquatic Toxicology. 234:105796 |
| ISSN: | 0166-445X |
| Popis: | This study leveraged the Japanese medaka fish embryo model for the assessment of effects of select contaminants on early development in fish. Fish embryos were exposed to various pharmaceutical contaminants including synthetic hormones and non-steroidal anti-inflammatory drugs and their effects on development were observed. Initial screening determined that swim bladder inflation failure was the most common endpoint detected. Swim bladder inflation failure was first explored in a study demonstrating that medaka require access to the air-water interphase to inflate their swim bladders in a time-dependent manner, and swim bladder inflation failure was correlated with mortality. Fish embryos were exposed 24-hours post fertilization until hatch to concentration ranges of various pharmaceutical contaminants including: 17β-estradiol, 17α-ethinylestradiol, and levonorgestrel (1 to 1000 µg/L), or diclofenac (0.32 to 100 mg/L). The main effect observed across all four compounds was a significant increase in failure of swim bladder inflation with increasing exposure concentration (24 to 72-hours post-hatch). Following single compound experiments combinatorial exposures using no-observed-effect concentrations were conducted. The main effect observed was a significant decrease in inflation success 24-hours post-hatch following a binary mixture of levonorgestrel and 17α-ethinylestradiol, as well as a significant decrease in swim bladder inflation success at all times following exposure to a quaternary mixture of all four compounds. This study demonstrated that embryonic exposure to pharmaceutical compounds, both alone and in combination, resulted in failure of swim bladder inflation in larval Japanese medaka. |
| Databáze: | OpenAIRE |
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