Molecular genetic studies in Saudi population; identified variants from GWAS and meta-analysis in stroke
| Autor: | Haifa Abdulaziz Al-Basheer, A.M. Al-Sulaiman, Imran Ali Khan, Naelah Abdullah Alghamdi, Khalid Khalaf Alharbi, Raid Al-Baradie, Mohammad Abdullah Alotaibi, Abdullah Saud Aloyaid |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Population Meta-analysis studies Genome-wide association study Single-nucleotide polymorphism Saudi population Genome-wide association studies Article 03 medical and health sciences 0302 clinical medicine Internal medicine Genotype medicine education lcsh:QH301-705.5 Genetic association Genetics education.field_of_study business.industry Odds ratio Heritability Stroke 030104 developmental biology lcsh:Biology (General) Meta-analysis General Agricultural and Biological Sciences business 030217 neurology & neurosurgery |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 25, Iss 1, Pp 83-89 (2018) |
| ISSN: | 1319-562X |
| Popis: | Introduction: Stroke is a multifactorial and heterogeneous disorder, correlates with heritability and considered as one of the major diseases. The prior reports performed the variable models such as genome-wide association studies (GWAS), replication, case-control, cross-sectional and meta-analysis studies and still, we lack diagnostic marker in the global world. There are limited studies were carried out in Saudi population, and we aim to investigate the molecular association of single nucleotide polymorphisms (SNPs) identified through GWAS and meta-analysis studies in stroke patients in the Saudi population. Methods: In this case-control study, we have opted gender equality of 207 cases and 207 controls from the capital city of Saudi Arabia in King Saud University Hospital. The peripheral blood (5 ml) sample will be collected in two different vacutainers, and three mL of the coagulated blood will be used for lipid analysis (biochemical tests) and two mL will be used for DNA analysis (molecular tests). Genomic DNA will be extracted with the collected blood samples, and specific primers will be designed for the opted SNPs (SORT1-rs646218 and OLR1-rs11053646 polymorphisms) and PCR-RFLP will be performed and randomly DNA sequencing will be carried out to cross check the results. Results: The rs646218 and rs11053646 polymorphisms were significantly associated with allele, genotype and dominant models with and without crude odds ratios (OR’s) and Multiple logistic regression analysis (p |
| Databáze: | OpenAIRE |
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