Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices
| Autor: | Ryuichiro Suzuki, Yoshiko Masuda, Taisei Kanamoto, Yoshiharu Matahira, Shigemi Terakubo, Hiroshi Sakagami, Katsuyoshi Sunaga, Hideki Nakashima, Mineko Tomomura, Akito Tomomura, Shigeru Amano, Satoshi Yokose, Yoshiaki Shirataki, Watanabe Hirofumi, Masaki Okawara, Kunihiko Fukuchi |
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| Rok vydání: | 2017 |
| Předmět: |
Cancer Research
Ethyl acetate Pharmacology Antiviral Agents 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neoplasms Cytochrome P-450 CYP3A Hexanes Humans Simplexvirus Cytotoxic T cell fractionation Cytotoxicity ethyl acetate extraction Cell Proliferation Bacteria CYP3A4 Pistacia biology Plant Extracts Mastic Resin HIV Biological activity biology.organism_classification Anti-Bacterial Agents antibacterial /anti-HIV 0104 chemical sciences Mastic 010404 medicinal & biomolecular chemistry chemistry 030220 oncology & carcinogenesis anti-HSV CYP3A4 inhibition tumor specificity Antibacterial activity Research Article |
| Zdroj: | In Vivo. 31:591-598 |
| ISSN: | 1791-7549 |
| Popis: | Background: Most previous mastic investigators have not considered its potent cytotoxicity that may significantly affect the interpretation of obtained data. In the present study, we re-evaluated several biological activities of mastic extracts, based on chemotherapeutic indexes. Materials and Methods: Pulverized mastic gum was extracted with n-hexane and then with ethyl acetate or independently with methanol or n-butanol. Tumor specificity (TS) of the extracts was determined by their cytotoxicity against human malignant and non-malignant cells. Antibacterial activity was determined by their cytotoxicity against bacteria and normal oral cells. Antiviral activity was determined by their protection of viral infection and cytotoxic activity. Cytochrome P-450 (CYP) 3A4 activity was measured by β-hydroxylation of testosterone. Results: Ethyl acetate extract showed slightly higher tumor specificity (TS=2.6) and one order higher antibacterial activity (selectivity index (SI)=0.813) than other extracts (TS=1.4-2.5; SI=0.030-0.063). All extracts showed no anti-human immunodeficiency virus (HIV) activity, but some anti-herpes simplex virus (HSV) activity, which was masked by potent cytotoxicity. They showed strong inhibitory activity against CYP3A4. Conclusion: Ethyl acetate extraction following the removal of cytotoxic and CYP3A4 inhibitory substances by n-hexane can enhance antitumor and antibacterial activity of mastic. open access journal from January 2017 / Rermission has been acquired by e-mail from publisher |
| Databáze: | OpenAIRE |
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