Bilayer thickness in unilamellar extruded 1,2-dimyristoleoyl and 1,2-dierucoyl phosphatidylcholine vesicles: SANS contrast variation study of cholesterol effect
| Autor: | José A. Teixeira, Daniela Uhríková, Pavol Balgavý, Mária Hanulová, J. Gallová |
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| Rok vydání: | 2004 |
| Předmět: |
Materials science
Macromolecular Substances Surface Properties Lipid Bilayers Neutron scattering Gyration chemistry.chemical_compound Colloid and Surface Chemistry Phosphatidylcholine Physical and Theoretical Chemistry Contrast variation Cholesterol Vesicle Bilayer Water Surfaces and Interfaces General Medicine Small-angle neutron scattering Neutron Diffraction Crystallography Models Chemical chemistry Phosphatidylcholines Dimyristoylphosphatidylcholine Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 38:11-14 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2004.07.012 |
| Popis: | Small-angle neutron scattering on extruded unilamellar vesicles in water was used to study bilayer thickness when cholesterol (CHOL) was added at 44.4 mol% to 1,2-dimyristoleoylphosphatidylcholine (diC14:1PC) and 1,2-dierucoylphosphatidylcholine (diC22:1PC) bilayers. Using the (1)H(2)O/(2)H(2)O contrast variation and the small-angle form of Kratky-Porod approximation, the bilayer gyration radii at infinite contrast R(g,infinity) and the bilayer thickness parameters d(g,infinity) = 12(0.5)R(g,infinity) were obtained at 30 degrees C. Addition of cholesterol to diC14:1PC increased the d(g,infinity) from 3.72 +/- 0.02 to 4.26 +/- 0.01 nm, while in the diC22:1PC bilayers the d(g,infinity) change observed was within the experimental error: +0.23 +/- 0.23 nm. |
| Databáze: | OpenAIRE |
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