Entry of amphotropic and 10A1 pseudotyped murine retroviruses is restricted in hematopoietic stem cell lines
| Autor: | Dorothee von Laer, Birgit Vogt, Carol Stocking, Wolfram Ostertag, Joachim Kruppa, Martina Donath, Silke Thomsen, Alan Rein |
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| Rok vydání: | 1998 |
| Předmět: |
viruses
Immunology Genetic Vectors Biology Microbiology CXCR4 Mice Virology Murine leukemia virus medicine Animals Humans Progenitor cell Gene Transfer Techniques Hematopoietic stem cell biology.organism_classification Hematopoietic Stem Cells Cell biology Virus-Cell Interactions Haematopoiesis medicine.anatomical_structure Retroviridae Insect Science Pseudotyping Stem cell K562 cells |
| Zdroj: | Journal of virology. 72(2) |
| ISSN: | 0022-538X |
| Popis: | Although transduction with amphotropic murine leukemia virus (MLV) vectors has been optimized successfully for hematopoietic differentiated progenitors, gene transfer to early hematopoietic cells (stem cells) is still highly restricted. A similar restriction to gene transfer was observed in the mouse stem cell line FDC-Pmix compared with transfer in the more mature myeloid precursor cell line FDC-P1 and the human erythroleukemia cell line K562. Gene transfer was not improved when the vector was pseudotyped with gp70 SU of the 10A1 strain of MLV, which uses the receptor of the gibbon ape leukemia virus (Pit1), in addition to the amphotropic receptor (Pit2). Although 10A1 and amphotropic gp70 SU bound to FDC-P1, K562, and fibroblasts, no binding to FDC-Pmix cells was detected. This indicates that FDC-Pmix cells lack functional Pit2 and Pit1 receptors. Pseudotyping with the vesicular stomatitis virus G protein improved transduction efficiency in FDC-Pmix stem cells by 2 orders of magnitude, to fibroblast levels, confirming a block to retroviral infection at the receptor level. |
| Databáze: | OpenAIRE |
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