Capillary isotachophoresis with ESI-MS detection: Methodology for highly sensitive analysis of ibuprofen and diclofenac in waters
| Autor: | Zdena Malá, Petr Boček, Petr Gebauer |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Analyte Diclofenac Capillary action Electrospray ionization Analytical chemistry Ibuprofen 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Limit of Detection medicine Environmental Chemistry Solid phase extraction Spectroscopy Detection limit Chromatography Isotachophoresis Chemistry Anti-Inflammatory Agents Non-Steroidal Solid Phase Extraction 010401 analytical chemistry Detector 021001 nanoscience & nanotechnology 0104 chemical sciences 0210 nano-technology Water Pollutants Chemical medicine.drug |
| Zdroj: | Analytica Chimica Acta. 907:1-6 |
| ISSN: | 0003-2670 |
| Popis: | The possibilities of reaching higher sensitivity in capillary electrophoretic analyses of complex samples with ESI-MS detection were investigated on the example of analysis of diclofenac and ibuprofen in waters. The applied separation approach is based on application of isotachophoresis that ensures permanent stacking of analytes until they reach the detector. Investigation of the possibilities of MS detector optimization have shown that optimization of fragmentor voltage and working in the SIM mode with collection of data for multiple fragments both increases the method specificity and approx. doubles its sensitivity. Combination with an offline SPE preconcentration step resulted in very high sensitivity of the described methodology with a reached LOD below 2 × 10(-12) M, corresponding to analyte levels of 0.6 ng L(-1) of diclofenac and 0.4 ng L(-1) of ibuprofen. The results demonstrate that CE-MS, particularly when performed in the ITP mode, has the potential to reach sensitivities comparable to HPLC-MS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect