Rapid and Reversible Nuclear Accumulation of Cytoplasmic tRNA in Response to Nutrient Availability
| Autor: | Rebecca L. Hurto, Hussam H. Shaheen, Michael L. Whitney, Anita K. Hopper |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Saccharomyces cerevisiae
Active Transport Cell Nucleus Aminoacylation RNA Transfer Gene expression medicine Amino Acids Molecular Biology In Situ Hybridization Fluorescence Cell Nucleus chemistry.chemical_classification biology Articles Cell Biology biology.organism_classification Cyclic AMP-Dependent Protein Kinases Carbon Amino acid Cell nucleus Glucose medicine.anatomical_structure chemistry Biochemistry Cytoplasm Transfer RNA Nucleus Signal Transduction |
| Zdroj: | Molecular Biology of the Cell. 18:2678-2686 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e07-01-0006 |
| Popis: | Cytoplasmic tRNAs have recently been found to accumulate in the nucleus during amino acid starvation in yeast. The mechanism and regulation by which tRNAs return to the nucleus are unclear. Here, we show accumulation of cytoplasmic tRNA in the nucleus also occurs during glucose starvation. Nuclear accumulation of tRNA in response to acute glucose or amino acid starvation is rapid, reversible, requires no new transcription, and is independent of the aminoacylation status of tRNA. Gradual depletion of nutrients also results in the accrual of tRNA in the nucleus. Distinct signal transduction pathways seem to be involved in the accumulation of cytoplasmic tRNA in the nucleus in response to amino acid versus glucose starvation. These findings suggest tRNA nucleocytoplasmic distribution may play a role in gene expression in response to nutritional stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|