Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries
| Autor: | Relja Beck, J. Stefanovska, Mariana Ionita, Dhimiter Rapti, M. F. Sommer, Zvezdelina Kirkova, Enstela Shukullari, Steffen Rehbein, Nemanja Zdravkovic, Ioan Liviu Mitrea, Cornelia Silaghi, Darka Hamel, Martin Knaus, Balázs Capári, Ana Vasić |
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| Rok vydání: | 2015 |
| Předmět: |
Giardiasis
Veterinary medicine Biology Feces Dogs parasitic diseases Animals Humans Parasite hosting Dog Diseases Europe Eastern Direct fluorescent antibody General Veterinary Glutamate dehydrogenase Giardia General Medicine Ribosomal RNA biology.organism_classification DNA extraction Infectious Diseases RNA Ribosomal Giardia duodenalis Insect Science Multilocus sequence typing Parasitology Giardia lamblia Dog Multilocus genotyping Assemblages South Eastern Europe Multilocus Sequence Typing |
| Zdroj: | Parasitology Research. 114:2165-2174 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Giardia duodenalis is a worldwide occurring proto- zoan that can infect various mammalian hosts. While living con- ditions are getting closer between pet animals and owners, there is discussion whether dogs may contribute to the transmission of these pathogens to humans. The present study was conducted in order to identify the Giardia assemblages in dogs from South Eastern Europe. For this purpose, 1645 faecal samples of house- hold and shelter dogs from Albania, Bulgaria, Hungary, Macedonia, Romania and Serbia were tested for Giardia coproantigen by enzyme-linked immunosorbent assay (ELISA). A subset of 107 faecal samples demonstrating Giardia cysts by direct immunofluorescence assay (IFA) or mi- croscopy (15-22 per country) plus 26 IFA-positive canine faecal samples from Croatia were used for DNA extraction and multilocus sequence typing with nested PCRs targeting five dif- ferent gene loci: SSU rRNA, ITS1-5.8S-ITS2, beta giardin (bg), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi). One third (33.7 %) of the samples tested positive for Giardia antigen in the coproantigen ELISA. Shelter dogs were infected more frequently than household dogs (57.2 vs. 29.7 %, p |
| Databáze: | OpenAIRE |
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