| Autor: |
Joussef-Pi��a, Samira, Nankya, Immaculate, Nalukwago, Sophie, Baseke, Joy, Rwambuya, Sandra, Winner, Dane, Kyeyune, Fred, Chervenak, Keith, Thiel, Bonnie, Asaad, Robert, Dobrowolski, Curtis, Luttge, Benjamin, Lawley, Blair, Kityo, Cissy M., Boom, W. Henry, Karn, Jonathan, Qui��ones-Mateu, Miguel E. |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.18482097.v1 |
| Popis: |
Additional file 7: Figure S6. Quantification of number of cells used in the EDITS assay. Cellular DNA was extracted from one million memory CD4+ T cells purified from 15 Ugandan and 25 U.S. patients, as well as one million Jurkat E4 (HIV+)/Jurkat E^-1 (HIV-) cells from five points of the standard curve. One microlliter of cellular DNA was use to PCR amplify a 197 bp fragment of the cellular albumin gene. Amplicons were purified and quantified using Qubit 2.0 (Thermo Fisher Scientific). Unpaired t test was used to compare the concentration of albumin amplicon (ng/ul) between both cohort of patients and the cell mixture from the standard curve. Median values and interquartile range are depicted. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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