Semiquantitative Dot Blot with the GRA8 antigen to differentiate the stages of toxoplasmosis infection
| Autor: | Juan Gabriel Costa, María Julia Vilariño |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030231 tropical medicine Immunoblotting Protozoan Proteins Dot blot Antibodies Protozoan Antigens Protozoan Enzyme-Linked Immunosorbent Assay Microbiology Sensitivity and Specificity law.invention 03 medical and health sciences 0302 clinical medicine Thioredoxins Antigen law medicine Technique development Humans Serologic Tests Cloning Molecular Molecular Biology biology Escherichia coli Proteins Toxoplasma gondii Specific igg biology.organism_classification medicine.disease Virology Toxoplasmosis Recombinant Proteins 030104 developmental biology Immunoglobulin G biology.protein Recombinant DNA Antibody Toxoplasma |
| Zdroj: | Journal of microbiological methods. 149 |
| ISSN: | 1872-8359 |
| Popis: | In this work we present a novel methodology to differentiate the phases of toxoplasmosis infection: the "semiquantitative Dot Blot". It is a simple technique that does not require expensive equipment, does not involve a long technique development, and can be used in a low-complexity laboratory. In this study, two recombinant sequences of Toxoplasma gondii GRA8 antigen were used, and specific IgG antibodies were detected in selected patient samples. This method makes it possible to obtain a score for each serum and define whether the patient is in the acute or chronic phase of the infection. The sensitivity and specificity results varied depending on the antigenic sequence used. With GRA8A, 62.1% and 72.7% were obtained, while with GRA8B, 82.8% and 72.1% were obtained, respectively. Although the sensitivity and specificity values were not close to 100%, they were similar to those reported with the same antigens in ELISA. Therefore, this quantitative technique would be a good alternative to ELISA. |
| Databáze: | OpenAIRE |
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