Atrazine degradation by encapsulated Rhodococcus erythropolis NI86/21
| Autor: | K. Jury, Tony Vancov, L. Van Zwieten |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Powdered activated carbon treatment
food.ingredient Alginates Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound food Skimmed milk Rhodococcus Soil Pollutants Atrazine Cell encapsulation Microbial inoculant Soil Microbiology Chromatography biology Herbicides General Medicine biology.organism_classification Microspheres chemistry Delayed-Action Preparations Bentonite Water Microbiology Soil microbiology Bacteria Water Pollutants Chemical Biotechnology |
| Zdroj: | Journal of applied microbiology. 99(4) |
| ISSN: | 1364-5072 |
| Popis: | Aims: To develop an encapsulation procedure for Rhodococcus erythropolis NI86/21 and demonstrate its use as a slow-release inoculant for reducing atrazine levels in aquatic and terrestrial environments. Methods and Results: Alginate encapsulation procedures were developed for the atrazine-degrading bacteria R. erythropolis NI86/21. Several bead amendments, including bentonite, powdered activated carbon (PAC) and skimmed milk (SM), were evaluated for slow release of R. erythropolis NI86/21 and efficacy of atrazine degradation. All bead types demonstrated a capacity to degrade atrazine in basal minimal nutrient buffer whilst continually releasing viable bacterial cells. We found that the addition of bentonite hastened cell release whilst SM sustained cell viability in bead formulations. Reducing the percentage of SM to 1% (w/v) resulted in faster rates of atrazine degradation in both liquid and soil, and was found to prolong cell survival upon bead storage. Limited oxygen transfer affects the capacity of the encapsulated R. erythropolis cells to degrade atrazine. Conclusions: Degradation studies have demonstrated the efficacy of R. erythropolis encapsulated cells to degrade atrazine in amended liquid and soil. However, in their current formulation, the wet alginate-based beads are impractical for field application because of their poor cell viability during storage. Significance and Impact of the Study: R. erythropolis NI86/21-encapsulated cells have the potential to reduce atrazine residues in a number of soil and water environments, possibly ensuring the continued registration and use of atrazine in agriculture by minimizing or eliminating nontarget effects. |
| Databáze: | OpenAIRE |
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