Purification and Biochemical Characterization of Sucrose synthase from the Stem of Nettle (Urtica dioica L.)
| Autor: | Giampiero Cai, Gea Guerriero, Lavinia Mareri, Jean-Francois Hausman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose 01 natural sciences Catalysis Inorganic Chemistry Cell wall lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Urtica dioica Sucrose synthase bast fibers protein purification phosphorylation Protein purification Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy biology Organic Chemistry Callose General Medicine Plant cell Computer Science Applications 030104 developmental biology chemistry Biochemistry lcsh:Biology (General) lcsh:QD1-999 Bast fibre biology.protein 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 851, p 851 (2021) International Journal of Molecular Sciences Volume 22 Issue 2 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Sucrose synthase is a key enzyme in sucrose metabolism as it saves an important part of sucrose energy in the uridine-5&prime diphosphate glucose (UDP-glucose) molecule. As such it is also involved in the synthesis of fundamental molecules such as callose and cellulose, the latter being present in all cell walls of plant cells and therefore also in the gelatinous cell walls of sclerenchyma cells such as bast fibers. Given the importance of these cells in plants of economic interest such as hemp, flax and nettle, in this work we have studied the occurrence of Sucrose synthase in nettle stems by analyzing its distribution between the cytosol, membranes and cell wall. We have therefore developed a purification protocol that can allow the analysis of various characteristics of the enzyme. In nettle, Sucrose synthase is encoded by different genes and each form of the enzyme could be subjected to different post-translational modifications. Therefore, by two-dimensional electrophoresis analysis, we have also traced the phosphorylation profile of Sucrose synthase isoforms in the various cell compartments. This information paves the way for further investigation of Sucrose synthase in plants such as nettle, which is both economically important, but also difficult to study. |
| Databáze: | OpenAIRE |
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