General purpose lamps induce polyoma DNA replication in H3 cells
| Autor: | Susan E. Rutberg, Ze'ev Ronai |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA Replication
Ultraviolet Rays viruses Virus Integration Biology Virus Replication Biochemistry Virus law.invention Cell Line chemistry.chemical_compound law Replication (statistics) medicine Animals Physical and Theoretical Chemistry Fibroblast Lighting Incandescent light bulb DNA replication General Medicine Fibroblasts Virology Cell biology Rats Mercury-vapor lamp Blotting Southern Halogen lamp medicine.anatomical_structure chemistry DNA Viral Polyomavirus DNA |
| Zdroj: | Photochemistry and photobiology. 58(2) |
| ISSN: | 0031-8655 |
| Popis: | — We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount of UV radiation emitted by three different light sources was determined and the effects of each source on the replication of polyoma DNA was assessed. Our findings indicate that a 100 W incandescent lamp had a minimal effect on replication, whereas a 90 s exposure to a halogen lamp or a 160 W mercury vapor lamp induced replication 1.5-fold and 2-fold, respectively, in comparison with nontreated controls. We have previously shown that asynchronous polyoma replication in H3 cells involves UV-inducible cellular protein factors. Our present results indicate that these factors are also activated by exposure to commonly used lamps that emit comparable doses of UV radiation. |
| Databáze: | OpenAIRE |
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