A novel carbon tipped single micro-optrode for combined optogenetics and electrophysiology

Autor: Lili Veronika Nagy, Dénes Budai, István Hernádi, Nóra Henn-Mike, Zoltán Kónya, Zsolt Kristóf Bali, Dániel Madarász, Csaba Varga, Balázs Márki, Attila D. Vizvári
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Optical fiber
Dopamine
Action Potentials
lcsh:Medicine
Biosensing Techniques
Local field potential
law.invention
Micrometre
0302 clinical medicine
Animal Cells
law
Medicine and Health Sciences
Electric Impedance
Fiber Optic Technology
03.02. Klinikai orvostan
lcsh:Science
Neurons
Brain Mapping
Microscopy
Multidisciplinary
Chemical Reactions
Light Microscopy
Brain
Optical Devices
Equipment Design
03.05. Egyéb orvostudományok
Fibers
Chemistry
Bioassays and Physiological Analysis
Physical Sciences
Calibration
Engineering and Technology
Cellular Types
Artifacts
Research Article
Materials science
Materials by Structure
Amorphous Solids
Materials Science
Genetic Vectors
Surgical and Invasive Medical Procedures
Optogenetics
Research and Analysis Methods
03 medical and health sciences
Channelrhodopsins
Carbon Fiber
Oxidation
Animals
Rats
Wistar
Genetically modified animal
Electrodes
Functional Electrical Stimulation
lcsh:R
Biology and Life Sciences
01.06. Biológiai tudományok
Neurophysiological Analysis
Cell Biology
Carbon
Amperometry
Microelectrode
Electrophysiology
030104 developmental biology
Cellular Neuroscience
Microscopy
Electron
Scanning
lcsh:Q
Glass
Electronics
Microelectrodes
030217 neurology & neurosurgery
Neuroscience
Biomedical engineering
Zdroj: PLoS ONE, Vol 13, Iss 3, p e0193836 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Optical microelectrodes (optrodes) are used in neuroscience to transmit light into the brain of a genetically modified animal to evoke and record electrical activity from light-sensitive neurons. Our novel micro-optrode solution integrates a light- transmitting 125 micrometer optical fiber and a 9 micrometer carbon monofilament to form an electrical lead element, which is contained in a borosilicate glass sheathing coaxial arrangement ending with a micrometer-sized carbon tip. This novel unit design is stiff and slender enough to be used for targeting deep brain areas, and may cause less tissue damage compared with previous models. The center-positioned carbon fiber is less prone to light-induced artifacts than side-lit metal microelectrodes previously presented. The carbon tip is capable of not only recording electrical signals of neuronal origin but can also provide valuable surface area for electron transfer, which is essential in electrochemical (voltammetry, amperometry) or microbiosensor applications. We present details of design and manufacture as well as operational examples of the newly developed single micro- optrode, which includes assessments of 1) carbon tip length–impedance relationship, 2) light transmission capabilities, 3) photoelectric artifacts in carbon fibers, 4) responses to dopamine using fast-scan cyclic voltammetry in vivo, and 5) optogenetic stimulation and spike or local field potential recording from the rat brain transfected with channelrhodopsin-2. With this work, we demonstrate that our novel carbon tipped single micro-optrode may open up new avenues for use in optogenetic stimulation when needing to be combined with extracellular recording, electrochemical, or microbiosensor measurements performed on a millisecond basis.
Databáze: OpenAIRE
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