Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus
| Autor: | John H. Morrison, Patrick R. Hof, William G.M. Janssen, Diana Moga, Prabhakar Vissavajjhala, Thomas M. Moran, Sharon M. Czelusniak |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Calbindins Interneuron Hippocampus AMPA receptor Biology Neurotransmission Hippocampal formation Calbindin Rats Sprague-Dawley Mice S100 Calcium Binding Protein G Interneurons Postsynaptic potential Neural Pathways medicine Animals Receptors AMPA Autoantibodies Mice Inbred BALB C Pyramidal Cells musculoskeletal neural and ocular physiology General Neuroscience Neural Inhibition Immunohistochemistry Rats Up-Regulation Cell biology Microscopy Electron Parvalbumins medicine.anatomical_structure nervous system Calbindin 2 Synapses biology.protein Encephalitis Neuroscience Parvalbumin |
| Zdroj: | The Journal of Comparative Neurology. 462:15-28 |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.10710 |
| Popis: | Rasmussen's encephalitis is a childhood disease resulting in intractable seizures associated with hippocampal and neocortical inflammation. An autoantibody against the GluR3 subunit of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors is implicated in the pathophysiology of Rasmussen's encephalitis. AMPA receptors mediate excitatory neurotransmission in the brain and contain combinations of four subunits (GluR1-4). Although the distributions of GluR1, GluR2, and GluR4 are known in some detail, the cellular distribution of GluR3 in the mammalian brain remains to be described. We developed and characterized a GluR3-specific monoclonal antibody and quantified the cellular distribution of GluR3 in CA1 of the rat hippocampus. GluR3 immunoreactivity was detected in all pyramidal neurons and astrocytes and in most interneurons. We quantified the intensity of GluR3 immunoreactivity in interneuron subtypes defined by their calcium-binding protein content. GluR3 immunofluorescence, but not GluR1 or GluR2 immunofluorescence, was significantly elevated in somata of parvalbumin-containing interneurons compared to pyramidal somata. Strikingly, increased GluR3 immunofluorescence was not observed in calbindin- and calretinin-containing interneurons. Furthermore, 24% of parvalbumin-containing interneurons could be distinguished from surrounding neurons based on their intense GluR3 immunoreactivity. This subpopulation had significantly elevated GluR3 immunoreactivity compared to the rest of parvalbumin-containing interneurons. Electron microscopy revealed enriched GluR3 immunoreactivity in parvalbumin-containing perikarya at cytoplasmic and postsynaptic sites. Parvalbumin-containing interneurons, potent inhibitors of cortical pyramidal neurons, are vulnerable in the brains of epileptic patients. Our findings suggest that the somata of these interneurons are enriched in GluR3, which may render them vulnerable to pathological states such as epilepsy and Rasmussen's encephalitis. |
| Databáze: | OpenAIRE |
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