Towards An Advanced Graphene-Based Magnetic Resonance Imaging Contrast Agent: Sub-acute Toxicity and Efficacy Studies in Small Animals
| Autor: | Kenneth R. Shroyer, Shruti Kanakia, Dung Minh Hoang, Jimmy Toussaint, William Moore, Youssef Zaim Wadghiri, Stephen Lee, Balaji Sitharaman, Sayan Mullick Chowdhury |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Dose Gadolinium Contrast Media chemistry.chemical_element 02 engineering and technology Article 030218 nuclear medicine & medical imaging law.invention Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law medicine Animals Rats Wistar Manganese Multidisciplinary medicine.diagnostic_test Chemistry Graphene business.industry Magnetic resonance imaging 021001 nanoscience & nanotechnology Magnetic Resonance Imaging Orders of magnitude (mass) Rats 3. Good health Dextran Toxicity Nanoparticles Female Graphite Molecular imaging 0210 nano-technology Nuclear medicine business |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep17182 |
| Popis: | Current clinical Gd3+-based T1 magnetic resonance imaging (MRI) contrast agents (CAs) are suboptimal or unsuitable, especially at higher magnetic fields (>1.5 Tesla) for advanced MRI applications such as blood pool, cellular and molecular imaging. Herein, towards the goal of developing a safe and more efficacious high field T1 MRI CA for these applications, we report the sub-acute toxicity and contrast enhancing capabilities of a novel nanoparticle MRI CA comprising of manganese (Mn2+) intercalated graphene nanoparticles functionalized with dextran (hereafter, Mangradex) in rodents. Sub-acute toxicology performed on rats intravenously injected with Mangradex at 1, 50 or 100 mg/kg dosages 3 times per week for three weeks indicated that dosages ≤50 mg/kg could serve as potential diagnostic doses. Whole body 7 Tesla MRI performed on mice injected with Mangradex at a potential diagnostic dose (25 mg/kg or 455 nanomoles Mn2+/kg; ~2 orders of magnitude lower than the paramagnetic ion concentration in a typical clinical dose) showed persistent (up to at least 2 hours) contrast enhancement in the vascular branches (Mn2+ concentration in blood at steady state = 300 ppb, per voxel = 45 femtomoles). The results lay the foundations for further development of Mangradex as a vascular and cellular/ molecular MRI probe. |
| Databáze: | OpenAIRE |
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