Obestatin and ghrelin in obese and in pregnant women
| Autor: | James E. deVente, Edward R. Seidel, Elizabeth Fontenot |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Physiology Radioimmunoassay Adipose tissue Receptors Cell Surface Biology Biochemistry Body Mass Index Cohort Studies Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Pregnancy Orexigenic Internal medicine Adipocyte medicine Humans Obesity RNA Messenger Receptor DNA Primers Base Sequence digestive oral and skin physiology Obestatin Immunohistochemistry Glucagon-like peptide-1 Ghrelin chemistry Peptide YY Female medicine.drug |
| Zdroj: | Peptides. 28:1937-1944 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2007.08.005 |
| Popis: | We identified, through qPCR, receptor mRNA for a number of gut peptides in female human omental fat: the incretins, GIP and GLP-1, the orexigenic peptides PYY-Y1 and -Y2 and ghrelin, and the anorexigenic peptide obestatin. Four cohorts of women were examined: lean controls (BMI23), obese (BMI41), obese diabetic and term pregnant women. Human fat expressed receptor mRNAs for all six peptides. Pregnant women expressed roughly three times as much orphan GPR-39 receptor, a proposed obestatin receptor, than other women and less than half as much of the ghrelin receptor (GHSR-1a). An immunoblot probed with a GPR-39 selective antibody yielded a single band corresponding to the correct molecular weight (52 kDa) for the proposed obestatin receptor. Fluorescent immunohistochemistry of human fat employing the same antibody indicated the receptor protein was localized to the adipocyte cell membrane. The concentration of obestatin circulating in blood was measured in the same cohort of women and was significantly lower in obese and obese diabetic women compared to control. |
| Databáze: | OpenAIRE |
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