A novel phosphotransferase system ofStreptococcus mutansis responsible for transport of carbohydrates with α-1,3 linkage
| Autor: | Dragana Ajdic, Zhiyun Chen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Sucrose Transcription Genetic Immunology Protein Array Analysis Nigerose Disaccharide Fructose macromolecular substances Biology Disaccharides Microbiology Bacterial Adhesion Article Streptococcus mutans Open Reading Frames chemistry.chemical_compound Operon otorhinolaryngologic diseases Extracellular Humans Phosphoenolpyruvate Sugar Phosphotransferase System Glucans General Dentistry Bacteriological Techniques Gene Expression Profiling Polysaccharides Bacterial Biofilm Dextranase PEP group translocation Metabolism Carbohydrate biology.organism_classification carbohydrates (lipids) stomatognathic diseases Glucose Hexosyltransferases chemistry Biochemistry Glucosyltransferases Biofilms Mutation bacteria |
| Zdroj: | Molecular Oral Microbiology. 28:114-128 |
| ISSN: | 2041-1006 |
| DOI: | 10.1111/omi.12009 |
| Popis: | The most common type of carbohydrate-transport system in Streptococcus mutans is the phosphoenolpyruvate (PEP)-sugar phosphotransferase system (PTS). We previously showed that fourteen PTSs exist in S. mutans UA159 (Ajdic et al., 2002). Several studies have shown that microorganisms growing in biofilms express different genes as compared to their planktonic counterparts. In this study, we showed that one PTS of S. mutans was expressed in sucrose-grown biofilms. Furthermore, the same PTS was also responsible for the transport and metabolism of disaccharide nigerose (3-O-α-D-glucopyranosyl-D-glucose). Additionally, the results indicate that the studied PTS might be involved in the transport and metabolism of carbohydrates synthesized by glucosyltransferase B (GtfB) and glucosyltransferase C (GtfC) of S. mutans. To our knowledge, this is the first report that shows PTS transport of a disaccharide (and possibly extracellular oligosaccharides) with α-1,3 linkage. |
| Databáze: | OpenAIRE |
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