Enzyme-free amplified SERS immunoassay for the ultrasensitive detection of disease biomarkers

Autor:	Wei Ma, Dan Guo, Da-Wei Li, Xuan-Xuan Zhang, Huanxing Han, Duo Xu
Rok vydání:	2020
Předmět:	Silver Metal Nanoparticles Enzyme free 02 engineering and technology Spectrum Analysis Raman Proof of Concept Study 01 natural sciences Catalysis Silver nanoparticle Double-Blind Method Limit of Detection Materials Chemistry medicine Humans Disease biomarker Immunoassay Detection limit Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry Metals and Alloys Reproducibility of Results General Chemistry 021001 nanoscience & nanotechnology Serum samples 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Ceramics and Composites Spectrum analysis 0210 nano-technology Biomarkers
Zdroj:	Chemical Communications. 56:2933-2936
ISSN:	1364-548X 1359-7345
DOI:	10.1039/c9cc09379f
Popis:	We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker α-fetoprotein with the detection limit of 3.3 × 10-13 g mL-1 and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::418933dd0ca0f32046edf2b2c1e741ce https://doi.org/10.1039/c9cc09379f Zobrazit plný text záznamu