Estudio del efecto inhibitorio de extractos de Salvia scutellarioides sobre la actividad de la enzima convertidora de angiotensina
| Autor: | Raúl Arenas-Carvajal, Gina Méndez-Callejas, Antonio Guzmán-Avendaño, Erica Pachón-Gómez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
arterial hypertension Multidisciplinary Chromatography Ethanol Salvia scutellarioides biology enzymatic activity Ethyl acetate Glycoside angiotensin II biology.organism_classification chemistry.chemical_compound Column chromatography Enzyme angiotensin-converting enzyme chemistry Petroleum ether Diethyl ether lcsh:Science (General) lcsh:Q1-390 |
| Zdroj: | Universitas Scientiarum, Vol 14, Iss 2-3, Pp 141-150 (2009) |
| ISSN: | 2027-1352 0122-7483 |
| Popis: | Study of the inhibitory effect of Salvia scutellarioides extracts on the activity of the angiotensin-converting enzyme. Objective. To identify groups of secondary metabolites of Salvia scutellarioides present in the fraction with the greatest inhibitory effect on the activity of the angiotensin-converting enzyme (ACE). Materials and methods. Dry plant material was used to prepare ethanolic extracts that were then concentrated and separated by column chromatography using solvents of different polarity (petroleum ether, dichloromethane , diethyl ether and ethanol). Subsequently, lung tissue isolated from Wistar rats was broken and centrifuged in order to separate the sol uble material. Proteins found in the supernatant were separated using a Sephacryl column; a pool was made with the fractions that showed activ ity with hippuryl-L-histidyl-L-leucine (HHL), the substrate of the ACE. This enzyme extract was used to measure the effect of plant ext racts obtained from S. scutellarioides on the ACE activity. Results: The fraction of ethyl acetate (T2) showed a greater inhibitory effect on the ACE activity. Metabolites found in T2 fraction were: tannins, cardiac glycosides, coumarins, and quinones. Conclusion. An antihypertensive effect was found for the plant species Salvia scutellarioides by studying the inhibitory effect on the ACE activity. Several groups of secondary metabolites present in the T2 fraction were identified, which could be responsible for this effect. |
| Databáze: | OpenAIRE |
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