MicroELISA method for measurement of human serum thymosin alpha 1
| Autor: | Paul H. Naylor, Milton G. Mutchnick, David F. Keren, Frederick E. Weller, Allan L. Goldstein |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
biology
Thymalfasin Chemistry Microchemistry Immunology Thymosin Dose-Response Relationship Immunologic Radioimmunoassay Temperature Enzyme-Linked Immunosorbent Assay Elisa assay Molecular biology Immunoenzyme Techniques Dose–response relationship Antibody Specificity Thymosin alpha biology.protein Immunology and Allergy Humans Antibody hormones hormone substitutes and hormone antagonists Thymosin α1 |
| Zdroj: | Journal of immunological methods. 80(1) |
| ISSN: | 0022-1759 |
| Popis: | A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring serum levels of thymosin alpha 1 and highly reproducible. The serum levels measured with the microELISA are comparable to serum levels of thymosin alpha 1 determined by the previously described radioimmunoassay for thymosin alpha 1. |
| Databáze: | OpenAIRE |
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