MPTH-09MOLECULAR MARKERS AND THEIR PROGNOSTIC IMPACT IN PEDIATRIC EPENDYMOMAS
| Autor: | Irene Slavc, Manila Antonelli, Johannes Gojo, Miklós Garami, Asuka Araki, Balazs Hegedus, József Virág, Zsuzsa Schaff, András Kiss, Monika Chocholous, Gábor Lotz, Christine Haberler, Thomas Czech |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Supratentorial Tumors Pathology medicine.medical_specialty business.industry Significant difference Extent of resection Fusion gene Text mining Oncology Patient age Immunohistochemistry Medicine Neurology (clinical) CNS TUMORS business Abstracts from the 20th Annual Scientific Meeting of the Society for Neuro-Oncology |
| Popis: | BACKGROUND: Ependymomas are the third most common CNS tumors in children. To date, extent of resection and patient age are the most important prognostic factors. To refine treatment strategies and improve patient outcome molecular markers are needed. Recently, the prognostic impact of tenascin-C (TNC) expression and gain of chromosome 1q have been described. Additionally, different molecular subgroups in supra-, infratentorial and spinal ependymomas have been identified including two subgroups characterized by RELA and YAP1 fusion genes in supratentorial cases. METHODS: We have analyzed a series of 75 pediatric ependymomas including 52 infra- and 23 supratentorial tumors. Patient age ranged between 0.9 and 21 years. FFPE sections were analysed using 1q25/1p36 FISH probes and antibodies against TNC, LAMA2, NF-κB, L1CAM and claudin-5. Immunohistochemical expression of LAMA2, NF-κB, L1CAM and claudin-5 was analyzed in additional 12 supratentorial ependymomas. RESULTS: 1q gain was found in 16%, TNC and Lama2 expression in 67% and 34%, respectively. Gain of 1q and TNC expression were predominantly detectable in infratentorial, whereas claudin-5 was almost exclusively expressed in supratentorial tumors. Claudin5, NF-κB and L1CAM expression was present in 68.6%, 48.6%, and 68.6% of all 35 supratentorial tumors, and a significant correlation between these markers was found. In two tumors with claudin5 and L1CAM expression RT-PCR verified the presence of a RELA fusion, thus confirming claudin5 and L1CAM as markers for tumors with RELA fusion. Patients with 1q gain and TNC expression had a significantly shorter overall survival, whereas no prognostic impact for LAMA2 and claudin5/L1CAM/ NF-κB was found. CONCLUSIONS: We confirm the prognostic impact of 1q gain and TNC expression in pediatric ependymomas and show that supratentorial ependymomas with RELA fusion can be recognized by L1CAM/claudin-5 expression. Nevertheless, there was no significant difference in outcome between supratentorial tumors with and without RELA fusion. |
| Databáze: | OpenAIRE |
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