Positive effect of silymarin on cell growth and differentiation in bovine and murine mammary cells
| Autor: | Eugenio Martignani, M. Motta, L. Starvaggi Cucuzza, Paolo Accornero, Michael V. Baratta, Elisabetta Macchi, Silvia Miretti |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Cellular differentiation Mammary gland Cell Enlargement Pharmacology Biology Antioxidants Cell Line Mice Mammary Glands Animal Food Animals Internal medicine medicine Animals Viability assay Protein kinase B Caspase 3 Cell growth Caseins Cell Differentiation Epithelial Cells Prolactin medicine.anatomical_structure Endocrinology Gene Expression Regulation Cell culture Apoptosis Cattle Female Animal Science and Zoology Signal Transduction Silymarin |
| Zdroj: | Journal of Animal Physiology and Animal Nutrition. 94:111-117 |
| ISSN: | 1439-0396 0931-2439 |
| DOI: | 10.1111/j.1439-0396.2008.00890.x |
| Popis: | Silymarin, a naturally acknowledged hepatoprotector used in humans to treat liver diseases has been tested in murine (HC11) and bovine (BME-UV) mammary epithelial cell lines to evaluate a possible direct effect on cell growth and differentiation in mammary gland. Silymarin enhanced cell proliferation (p < 0.05) from 10 to 1000 ng/ml in association with growth factors, (up to 20%) or alone (up to 15%) versus controls. Furthermore, silymarin (100 ng/ml) was able to increase (p < 0.05) beta-casein gene expression alone or in association with prolactin (5 microg/ml). These effects may be related with protein kinase B (AKT) activation induced by silymarin treatment (p < 0.05) and/or by a dose-related inhibitory effect (p < 0.05) on caspase-3 activity related to a protective role in cell apoptosis. These data suggest that silymarin should be considered a candidate to support mammary gland activity during a lactogenetic state. |
| Databáze: | OpenAIRE |
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