Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells
| Autor: | Akintade Dare, R. Hachisu, Tomohiro Okano, Satoshi Yokose, Shusaku Yoshiki, Akira Yamaguchi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
0301 basic medicine
Cell type Cell Survival Cellular differentiation Cell Separation Ionizing radiation Cell Line Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals General Dentistry Analysis of Variance Osteoblasts business.industry Chemistry Cell growth Skull Osteoblast Cell Differentiation Dose-Response Relationship Radiation 030206 dentistry DNA Alkaline Phosphatase Molecular biology Clone Cells Rats Dose–response relationship 030104 developmental biology medicine.anatomical_structure Animals Newborn Cell culture Alkaline phosphatase Nuclear medicine business Cell Division |
| Zdroj: | Journal of dental research. 76(2) |
| ISSN: | 0022-0345 |
| Popis: | Diagnostic radiation for immediate post-surgical assessment of osseointegrated dental implants has been discouraged, due to the possibility of detrimental effects of ionizing radiation on healing and remodeling of bone. To assess this possibility, we investigated the effects of ionizing radiation on proliferation and differentiation of osteoblasts using osteoblast-like cells isolated from the calvariae of newborn rats (ROB) and a clonal osteoblastic cell line (MC3T3-E1). The cells were exposed on day 3 to a single dose of x-rays at either 40, 100, 400, or 4000 mGy, respectively, from a linear accelerator radiotherapeutic machine (Linac) or a 40-mGy dose from a diagnostic chest x-ray machine. The effects of radiation on cell growth and alkaline-phosphatase-specific (ALP) activity were evaluated at three-day intervals after irradiation up to day 12 in ROB cells, and evaluated at day 12 in MC3T3-E1 cells. At the culture end-point, the effects on formation of bone-like nodules were also evaluated in both ROB and MC3T3-E1 cells. Exposure of 4000 mGy differentially affected the two cell types. It inhibited cell growth and alkaline phosphatase activity in ROB cells, slightly increased alkaline phosphatase activity, and inhibited DNA content in MC3T3-E1 cells. This irradiation also strongly inhibited the formation of bone-like nodules in ROB cells. On the other hand, exposure of 40-, 100-, and 400-mGy (Linac) and 40-mGy (diagnostic quality) irradiation induced no significant changes in cell growth, alkaline phosphatase activity, and formation of bone-like nodules in ROB cells. These doses also induced no significant changes in DNA content and ALP activity in MC3T3-E1 cells. These results indicate that ionizing radiation at a single dose of up to 400 mGy induces no significant changes in cell growth and differentiation of osteoblast-like cells, at least in vitro. Higher radiation doses (4000 mGy) may exert different effects on cell proliferation and cell differentiation of osteoblasts, depending on the cell types affected. Thus, diagnostic radiation seems to have less effect on proliferation and differentiation of osteoblasts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|