Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus
| Autor: | Junhuan Xu, Kristen Willie, Wenshuang Xie, W. B. Moraes, Nitika Khatri, Dee Marie Marty, Lucy R. Stewart |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Crops Agricultural Potyvirus Virus-induced gene silencing (VIGS) Plant Science Multigene silencing Genes Plant 01 natural sciences Marker gene Zea mays Plant Viruses 03 medical and health sciences Rhopalosiphum padi Gene Expression Regulation Plant Plant virus Gene expression Gene silencing Gene Silencing Gene Disease Resistance Ohio Plant Diseases Genetics Photobleaching biology Botany Vascular puncture inoculation (VPI) Maize dwarf mosaic virus biology.organism_classification 030104 developmental biology Genetic Techniques QK1-989 010606 plant biology & botany Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 21, Iss 1, Pp 1-17 (2021) |
| ISSN: | 1471-2229 |
| Popis: | Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of MDMV for both gene expression and gene silencing were examined. Results Infectious clones of an Ohio isolate of MDMV, MDMV OH5, were obtained, and engineered for gene expression only, and for simultaneous marker gene expression and virus-induced gene silencing (VIGS) of three endogenous maize target genes. Single gene expression in single insertion constructs and simultaneous expression of green fluorescent protein (GFP) and silencing of three maize genes in a double insertion construct was demonstrated. Constructs with GFP inserted in the N-terminus of HCPro were more stable than those with insertion at the N-terminus of CP in our study. Unexpectedly, the construct with two insertion sites also retained insertions at a higher rate than single-insertion constructs. Engineered MDMV expression and VIGS constructs were transmissible by aphids (Rhopalosiphum padi). Conclusions These results demonstrate that MDMV-based vector can be used as a tool for simultaneous gene expression and multi-gene silencing in maize. |
| Databáze: | OpenAIRE |
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