Steroid hormones and human choriogonadotropin influence the distribution of alpha6-integrin and desmoplakin 1 in gland-like endometrial epithelial spheroids
| Autor: | J Neulen, Rudolf E. Leube, Irmgard Classen-Linke, Volker U. Buck, Benjamin Rösing, M T Kohlen, A K Sternberg |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Histology Integrin Integrin alpha6 Human endometrium Chorionic Gonadotropin Adherens junction Extracellular matrix Endometrium 03 medical and health sciences 0302 clinical medicine Ishikawa cell line Spheroids Cellular medicine Humans Gonadal Steroid Hormones Cell adhesion Molecular Biology Cells Cultured 3D cell culture system Epithelial polarity Basement membrane Original Paper 030219 obstetrics & reproductive medicine biology Cell adhesion molecule Chemistry Desmoplakin Cell Biology Cell biology Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Endometrial receptivity Desmoplakins biology.protein Female |
| Zdroj: | Histochemistry and Cell Biology |
| ISSN: | 1432-119X 0948-6143 |
| Popis: | In human glandular endometrial epithelial cells, desmosomal and adherens junction proteins have been shown to extend from a subapically restricted lateral position to the entire lateral membrane during the implantation window of the menstrual cycle. Similarly, a menstrual cycle stage-dependent redistribution of the extracellular matrix adhesion protein α6-integrin has been reported. These changes are believed to be important for endometrial receptiveness and successful embryo implantation. To prove the hypothesis that steroid hormones and human choriogonadotropin can induce the redistribution of these adhesion molecules, we used the human endometrial cell line Ishikawa in a 3D culture system. Gland-like spheroids were grown in reconstituted basement membrane (Matrigel™). The lumen-bearing spheroids were treated for 2 or 4 days with ovarian steroids or human choriogonadotropin and then assessed by immunofluorescence microscopy. In addition, human endometrial biopsies were obtained from patients, who were in therapy for assisted reproductive technology, and were examined in parallel. Lateral redistribution of the desmosomal plaque protein desmoplakin 1 was observed in the spheroids treated either with progesterone, medroxyprogesterone acetate or human choriogonadotropin. Furthermore, the extracellular matrix adhesion protein α6-integrin showed an increased lateral membrane localization upon gestagen stimulation in the 3D culture system. The results of this study demonstrate that the 3D endometrial Ishikawa cell culture might be suited as an experimental model system to prove the effect of hormonal changes like those occurring during the window of implantation. Supplementary Information The online version contains supplementary material available at 10.1007/s00418-020-01960-z. |
| Databáze: | OpenAIRE |
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