Investigation of the effects of GABA receptor agonists in the differentiation of human induced pluripotent stem cells into dopaminergic neurons

Autor:	A. G. Kobylyansky, E. L. Arsenyeva, E. V. Novosadova, Sergey N. Illarioshkin, A. A. Zykova, N. F. Myasoedov, E. S. Manuilova, S A Antonov, M. A. Grefenstein, Igor A. Grivennikov
Rok vydání:	2016
Předmět:	0301 basic medicine Agonist Baclofen medicine.medical_specialty medicine.drug_class Cellular differentiation Induced Pluripotent Stem Cells Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine GABA receptor Internal medicine medicine Humans Calcium Signaling Induced pluripotent stem cell Receptor GABA Agonists Cells Cultured General Immunology and Microbiology Tyrosine hydroxylase Muscimol Dopaminergic Neurons Dopaminergic Cell Differentiation General Medicine Receptors GABA-A Cell biology 030104 developmental biology Endocrinology nervous system chemistry Calcium General Agricultural and Biological Sciences 030217 neurology & neurosurgery
Zdroj:	Doklady Biological Sciences. 470:244-246
ISSN:	1608-3105 0012-4966
Popis:	The influence of GABA receptor agonists on the terminal differentiation in vitro of dopaminergic (DA) neurons derived from IPS cells was investigated. GABA-A agonist muscimol induced transient elevation of intracellular Ca2+ level ([Ca2+] i ) in the investigated cells at days 5 to 21 of differentiation. Differentiation of cells in the presence of muscimol reduced tyrosine hydroxylase expression. Thus, the presence of active GABA-A receptors, associated with phenotype determination via Ca2+-signalling was demonstrated in differentiating human DA neurons.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::418051ba152913e387f813a0b6ce44c9 https://doi.org/10.1134/s0012496616050045 Zobrazit plný text záznamu Full text from SpringerLink