Genetic localization of the orevactaene/epipyrone biosynthetic gene cluster in Epicoccum nigrum
| Autor: | Hyung-Jin Kwon, Si-Hyung Park, Yoon Ji Lim, Eun Ha Choi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Clinical Biochemistry
Mutant Molecular Conformation Pharmaceutical Science 01 natural sciences Biochemistry Polyketide Ascomycota Transcription (biology) Polyketide synthase Drug Discovery Glycosyltransferase Gene cluster Molecular Biology Gene Pyrans biology 010405 organic chemistry Chemistry Organic Chemistry biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Multigene Family biology.protein Molecular Medicine Epicoccum nigrum |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 30:127242 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2020.127242 |
| Popis: | Epipyrone (EPN)-A (syn. orevactaene) is a polyketide compound of 3-d-galactosyl-4-hydroxy-2-pyrone with a modified heptaene acyl moiety, produced from Epicoccum nigrum and was reported to have various biological activities. Genome analysis identified a hypothetical EPN biosynthetic gene cluster (BGC) composed of the four genes epnABCD, which encode a highly-reducing fungal polyketide synthase, a glycosyltransferase, a cytochrome P450, and a transporter. The individual gene inactivation of epnABC resulted in the total loss of EPN production, while the inactivation of a nearby transcription factor-encoding gene had no effect on the production of EPN, substantiating that epnABCD is the EPN BGC. mRNA expression indicated no epnA transcription in the epnB knockout mutant and the occurrence of the bicistronic transcription of epnAB. This study defined an EPN BGC, which is the first blueprint reported for glycosylated 2-pyrone polyketide biosynthesis. |
| Databáze: | OpenAIRE |
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