Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse
| Autor: | Dorothy Li, Laura L. Johnson, Robert A. Steiner, Stephanie M. Krasnow, Anders Juréus, Donald K. Clifton, Matthew Cunningham, Dawit N. Teklemichael |
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| Předmět: |
Leptin
Male medicine.medical_specialty DNA Complementary Hypothalamus Nerve Tissue Proteins Biology chemistry.chemical_compound Mice Endocrinology Arcuate nucleus Reference Values Galanin-like peptide Internal medicine Complementary DNA medicine Animals Tissue Distribution Obesity RNA Messenger Cloning Molecular Messenger RNA GalP digestive oral and skin physiology Brain Mice Inbred C57BL chemistry Median eminence biology.protein Galanin-Like Peptide |
| Zdroj: | Karolinska Institutet |
| Popis: | Galanin-like peptide (GALP) is a newly discovered molecule whose expression in the brain is confined to the arcuate nucleus and median eminence. In the rat, cellular levels of GALP mRNA are reduced by fasting and reversed by peripheral administration of leptin. The purpose of this investigation was 1) to clone and map the distribution of GALP mRNA in the brain of the mouse; 2) to compare the pattern and magnitude of GALP mRNA expression in the leptin-deficient obese (ob/ob) mouse with that of wild-type controls; and 3) to examine the effects of leptin delivered into the brain on the expression of GALP mRNA in the ob/ob mouse. We report the sequence of a mouse GALP cDNA and show that GALP mRNA is expressed in the arcuate nucleus, median eminence, infundibular stalk, and the neurohypophysis of this species. The expression of GALP mRNA in the brain was markedly reduced in the ob/ob mice, compared with wild-type animals. Intracerebroventricular infusion of leptin to ob/ob mice increased both the number of GALP mRNA-expressing neurons and their content of GALP mRNA, compared with vehicle-treated controls. These observations demonstrate that GALP mRNA is induced by leptin through a direct action on the brain. |
| Databáze: | OpenAIRE |
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