K + /H + antiporter in alkaliphilic Bacillus sp. no. 66 (JCM 9763)
| Autor: | Toshiaki Kudo, Koki Horikoshi, Makio Kitada, Shinya Morotomi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Membrane potential
Quinine Efflux rate Chemistry Vesicle Antiporter Bacillus General Medicine Bacillus sp Hydrogen-Ion Concentration Microbiology Medicinal chemistry Antiporters Potassium-Hydrogen Antiporters Biochemistry Potassium Ph gradient Molecular Medicine Magnesium Membrane vesicle Protons Na+/K+-ATPase |
| Zdroj: | Extremophiles. 1:135-141 |
| ISSN: | 1433-4909 1431-0651 |
| DOI: | 10.1007/s007920050026 |
| Popis: | A K+/H+ antiport system was detected for the first time in right-side-out membrane vesicles prepared from alkaliphilic Bacillus sp. no. 66 (JCM 9763). An outwardly directed K+ gradient (intravesicular K+ concentration, K(in), 100 mM; extravesicular K+ concentration, K(out), 0.25 mM) stimulated uphill H+ influx into right-side-out vesicles and created the inside-acidic pH gradient (delta pH). This H+ influx was pH-dependent and increased as the pH increased from 6.8 to 8.4. Addition of 100 microM quinine inhibited the H+ influx by 75%. This exchange process was electroneutral, and the H+ influx was not stimulated by the imposition of the membrane potential (interior negative). Addition of K+ at the point of maximum delta pH caused a rapid K+-dependent H+ efflux consistent with the inward exchange of external K+ for internal H+ by a K+/H+ antiporter. Rb+ and Cs+ could replace K+ but Na+ and Li+ could not. The H+ efflux rate was a hyperbolic function of K+ and increased with increasing extravesicular pH (pH(out)) from 7.5 to 8.5. These findings were consistent with the presence of K+/H+ antiport activity in these membrane vesicles. |
| Databáze: | OpenAIRE |
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