Expression profiles of the Gα subunits during Xenopus tropicalis embryonic development
Autor: | Jaime Fuentealba, Soraya Villaseca, Cecilia Arriagada, Juan I Leal, María Victoria Hinrichs, Gabriela Toro-Tapia, Teresa Caprile, Juan Olate, Andréa Beyer, Alejandro Maureira, Marion Rodriguez, Marcela Torrejón |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Neural Tube G protein Xenopus Embryonic Development 03 medical and health sciences 0302 clinical medicine Heterotrimeric G protein Pleiotropism Genetics medicine Animals Amino Acid Sequence Molecular Biology In Situ Hybridization biology Embryogenesis Neural tube Gene Expression Regulation Developmental Cell Differentiation biology.organism_classification Heterotrimeric GTP-Binding Proteins Somite 030104 developmental biology medicine.anatomical_structure Somites Neural Crest Otic vesicle 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
Zdroj: | Gene Expression Pattern Artículos CONICYT CONICYT Chile instacron:CONICYT |
ISSN: | 1567-133X |
Popis: | Heterotrimeric G protein signaling plays major roles during different cellular events. However, there is a limited understanding of the molecular mechanisms underlying G protein control during embryogenesis. G proteins are highly conserved and can be grouped into four subfamilies according to sequence homology and function. To further studies on G protein function during embryogenesis, the present analysis identified four Gα subunits representative of the different subfamilies and determined their spatiotemporal expression patterns during Xenopus tropicalis embryogenesis. Each of the Gα subunit transcripts was maternally and zygotically expressed, and, as development progressed, dynamic expression patterns were observed. In the early developmental stages, the Gα subunits were expressed in the animal hemisphere and dorsal marginal zone. While expression was observed at the somite boundaries, in vascular structures, in the eye, and in the otic vesicle during the later stages, expression was mainly found in neural tissues, such as the neural tube and, especially, in the cephalic vesicles, neural crest region, and neural crest-derived structures. Together, these results support the pleiotropism and complexity of G protein subfamily functions in different cellular events. The present study constitutes the most comprehensive description to date of the spatiotemporal expression patterns of Gα subunits during vertebrate development. |
Databáze: | OpenAIRE |
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