Cloning and expression analysis of the transforming growth factor-beta receptors type 1 and 2 in the rainbow trout Oncorhynchus mykiss
| Autor: | Jose L. González Vecino, Tanja Maehr, Tiehui Wang, Christopher J. Secombes, Simon Wadsworth |
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| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Receptor complex TGF alpha Interferon Inducers Molecular Sequence Data Immunology Gene Expression Biology Internal medicine Leukocytes medicine Animals Amino Acid Sequence Cloning Molecular Receptor Cells Cultured Conserved Sequence Phylogeny R-SMAD Sequence Homology Amino Acid Macrophages Muscles Brain ACVRL1 Sequence Analysis DNA TGF beta receptor 2 Endoglin Head Kidney Cell biology Poly I-C Endocrinology Gene Expression Regulation Organ Specificity Oncorhynchus mykiss Signal transduction Receptors Transforming Growth Factor beta Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 37:115-126 |
| ISSN: | 0145-305X |
| Popis: | Transforming growth factor-β (TGF-β) binding to the TGF-β type I (TGFBR1) and type II (TGFBR2) receptors delivers a plethora of cell-type specific effects. Moreover, the responses to TGF-β are tuned by regulatory mechanisms at the receptor level itself. To further elucidate TGF-β family signal transduction in teleosts, we therefore cloned the first complete set of a putative TGF-β receptor complex in salmonids. Rainbow trout TGFBR1 and TGFBR2 are transmembrane proteins with a serine/threonine kinase domain and are highly conserved within vertebrates. High expression levels in muscle and brain indicate regulation of the TGF-β system in muscular and nervous systems. Lipopolysaccharide (LPS) induced expression of both receptor chains in RTgill cells while bacterial and viral mimics modulated the two receptors inversely in head kidney (HK) macrophages. In addition, T cell mitogens lowered receptor levels in HK leukocytes. These data provide the first insights into TGF-β type I and II receptor modulation during immune responses in teleost fish. |
| Databáze: | OpenAIRE |
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