Regulated redirection of central carbon flux enhances anaerobic production of bioproducts in Zymomonas mobilis
| Autor: | Julia I. Martien, Robert Landick, Yang Liu, Daniel Amador-Noguez, Yaoping Zhang, Indro Neil Ghosh |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Butanols Bioengineering Ethanol fermentation 01 natural sciences Applied Microbiology and Biotechnology Zymomonas mobilis Metabolic engineering 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Bioproducts Glycolysis Anaerobiosis Butylene Glycols 030304 developmental biology 0303 health sciences Zymomonas biology Isobutanol biology.organism_classification Carbon Glucose chemistry Biochemistry Microorganisms Genetically-Modified Flux (metabolism) Pyruvate decarboxylase Biotechnology |
| Zdroj: | Metabolic engineering. 61 |
| ISSN: | 1096-7184 |
| Popis: | The microbial production of chemicals and fuels from plant biomass offers a sustainable alternative to fossilized carbon but requires high rates and yields of bioproduct synthesis. Z. mobilis is a promising chassis microbe due to its high glycolytic rate in anaerobic conditions that are favorable for large-scale production. However, diverting flux from its robust ethanol fermentation pathway to nonnative pathways remains a major engineering hurdle. To enable controlled, high-yield synthesis of bioproducts, we implemented a central-carbon metabolism control-valve strategy using regulated, ectopic expression of pyruvate decarboxylase (Pdc) and deletion of chromosomal pdc. Metabolomic and genetic analyses revealed that glycolytic intermediates and NADH accumulate when Pdc is depleted and that Pdc is essential for anaerobic growth of Z. mobilis. Aerobically, all flux can be redirected to a 2,3-butanediol pathway for which respiration maintains redox balance. Anaerobically, flux can be redirected to redox-balanced lactate or isobutanol pathways with ≥65% overall yield from glucose. This strategy provides a promising path for future metabolic engineering of Z. mobilis. |
| Databáze: | OpenAIRE |
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