In vitro toxicity of amorphous silica nanoparticles in human colon carcinoma cells
| Autor: | Silvia Diabaté, Joanna Pelka, Doris Marko, Anne Frühmesser, H. Blank, Heike P. Schuchmann, Helge Gehrke, Clarissa Marquardt, Carsten Weiss, Dagmar Gerthsen, Lena L. Hecht, Melanie Esselen |
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| Rok vydání: | 2012 |
| Předmět: |
MAPK/ERK pathway
Materials science MAP Kinase Signaling System DNA damage Glutamate-Cysteine Ligase Blotting Western Biomedical Engineering Toxicology medicine.disease_cause Polymerase Chain Reaction HT29 Cells chemistry.chemical_compound medicine Humans Particle Size Cytotoxicity Cell Proliferation L-Lactate Dehydrogenase Glutathione Silicon Dioxide In vitro Mitochondria Comet assay Oxidative Stress chemistry Biochemistry Colonic Neoplasms Microscopy Electron Scanning Biophysics Nanoparticles RNA Comet Assay Reactive Oxygen Species Oxidative stress DNA Damage |
| Zdroj: | Nanotoxicology. 7:274-293 |
| ISSN: | 1743-5404 1743-5390 |
| DOI: | 10.3109/17435390.2011.652207 |
| Popis: | The use of nanostructured silica (SiO2) particles is no longer restricted to biomedical and (bio-) technological fields but rather finding applications in products of the food industry. Thus, our studies on the toxicological relevance of SiO2 nanoparticles focused on cytotoxic effects, the modulation of the cellular redox status and the impact on DNA integrity in human colon carcinoma cells (HT29). The results indicate that these SiO2 nanoparticles stimulate the proliferation of HT29 cells, depending on the incubation time and the particle size. The cytotoxicity of the investigated SiO2 nanoparticles was found to depend on the concentration, size and on the FCS content of the culture medium. Furthermore, SiO2 seem to interfere with glutathione biosynthesis. The results indicate further that effects of SiO2 NPs are not mediated by oxidative stress but by interference with the MAPK/ERK1/2 as well as the Nrf2/ARE signalling pathways. Additionally, investigations regarding DNA integrity revealed no substantial (oxidative) DNA damage. |
| Databáze: | OpenAIRE |
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