Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
Autor: | Adrien Thurotte, Frederic Amiard, Brigitte Veidl, Myriam Badawi, Frédérick Niepceron, Matteo Scarsini, Benoît Schoefs, Justine Marchand, Fabienne Lagarde |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
In situ
biology Metabolite lipid droplets Infrared spectroscopy Plant culture Phaeodactylum tricornutum Bohlin Plant Science biology.organism_classification SB1-1110 chemistry.chemical_compound FTIR spectroscopy Diatom chemistry Biochemistry Lipid droplet algal physiology Microscopy phytoplankton Phaeodactylum tricornutum Fourier transform infrared spectroscopy macromolecules quantification Original Research |
Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 12 (2021) |
ISSN: | 1664-462X |
Popis: | Diatoms are feedstock for the production of sustainable biocommodities, including biofuel. The biochemical characterization of newly isolated or genetically modified strains is seminal to identify the strains that display interesting features for both research and industrial applications. Biochemical quantification of organic macromolecules cellular quotas are time-consuming methodologies which often require large amount of biological sample. Vibrational spectroscopy is an essential tool applied in several fields of research. A Fourier transform infrared (FTIR) microscopy-based imaging protocol was developed for the simultaneous cellular quota quantification of lipids, carbohydrates, and proteins of the diatom Phaeodactylum tricornutum. The low amount of sample required for the quantification allows the high throughput quantification on small volume cultures. A proof of concept was performed (1) on nitrogen-starved experimental cultures and (2) on three different P. tricornutum wild-type strains. The results are supported by the observation in situ of lipid droplets by confocal and brightfield microscopy. The results show that major differences exist in the regulation of lipid metabolism between ecotypes of P. tricornutum. |
Databáze: | OpenAIRE |
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