Hunting for genes by functional screens
| Autor: | Endre Kiss-Toth, Eva E. Qwarnstrom, S K Dower |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Proteomics
Endocrinology Diabetes and Metabolism Immunology Genomics Computational biology Biology General Biochemistry Genetics and Molecular Biology DNA sequencing Cell Line Genes Reporter Protein Interaction Mapping Animals Humans Immunology and Allergy Serial analysis of gene expression Gene Genetics Expressed sequence tag Proteins Genes Mutagenesis RNA Interference Human genome Primary sequence Signal Transduction |
| Zdroj: | Cytokine & Growth Factor Reviews. 15:97-102 |
| ISSN: | 1359-6101 |
| DOI: | 10.1016/j.cytogfr.2004.02.002 |
| Popis: | Advances in high throughput sequencing technologies have led to an explosion of sequence information available for today's researchers. Efforts in the emerging next phase of the genomic era are focusing on the assignment of function to genes uncovered by genome sequencing programs. The main approaches include high throughput mutagenesis, predictions based on homology in primary sequence, microarray and proteomics. Despite the variety of strategies applied, only 30% of predicted human genes have any function assigned. There is a need, therefore, for additional tools to overcome some of the limitations of existing techniques. In this review we discuss some recent developments and their impact on gene function annotation, especially as they relate to the elucidation of signalling cascades activated by cytokines and growth factors. |
| Databáze: | OpenAIRE |
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