Molecular Cloning of Polymorphic Markers on RLGS Gel Using the Spot Target Cloning Method
| Autor: | Chikara Miyamoto, Yasushi Okazaki, Hidehiko Sugino, Christoph Plass, Masami Muramatsu, Hideo Shibata, Kenji Hirose, Veme M. Chapman, Shigeru Tamatsukuri, Nobuya Sasaki, Shinji Hirotsune, Hisato Okuizumi, Hirosumi Imoto, Yoshihide Hayashizaki, Yasuhiro Furuichi |
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| Rok vydání: | 1993 |
| Předmět: |
Genetic Markers
Restriction Mapping Restriction landmark genomic scanning Biophysics Molecular cloning Biology Biochemistry Genome Chromosomes Mice chemistry.chemical_compound Animals Cloning Molecular Deoxyribonucleases Type II Site-Specific Molecular Biology Cloning Genetics Polymorphism Genetic Spots Cell Biology Mice Inbred C57BL genomic DNA chemistry Mice Inbred DBA Genetic marker DNA |
| Zdroj: | Biochemical and Biophysical Research Communications. 194:1406-1412 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.1981 |
| Popis: | A new method for target cloning of DNA fragments corresponding to spots on the two-dimensional restriction landmark genomic scanning (RLGS) profile has been developed (targeted spot cloning). We used a Not I restriction trapper to select target DNA fragments from Not I, Eco RV double digests of genomic DNA. The use of the restriction trapper substantially reduces the background clones that are established from the direct recovery of RLGS spot DNA from the two-dimensional gels. Genomic DNA clones were isolated in this study as mouse genome markers for 58 spot loci that were previously characterized using RLGS spot mapping. This method provides a powerful tool for isolating DNA clones after their identification by RLGS system. |
| Databáze: | OpenAIRE |
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