Ordered progress of spermiogenesis to the fertilizable sperm of the medaka fish, Oryzias latipes, in cell culture
| Autor: | Eriko Takayama-Watanabe, Hiroyuki Abe, Kazuo Onitake, Makoto Suzuki, Akihiko Watanabe, Takahiro Sasaki |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Cytoplasm Somatic cell Spermiogenesis Oryzias Human fertilization Spermatocytes Animals Protamines Spermatogenesis Cells Cultured Genetics biology urogenital system Cell Differentiation Cell Biology biology.organism_classification Spermatids Spermatozoa Protamine Sperm Mitochondria Cell biology Kinetics Fertilization Sperm Tail biology.protein Mitochondrion localization Developmental Biology |
| Zdroj: | Development, Growth and Differentiation. 47:87-97 |
| ISSN: | 1440-169X 0012-1592 |
| DOI: | 10.1111/j.1440-169x.2005.00785.x |
| Popis: | Spermiogenesis is significant for producing sperm with equipment for achieving fertilization. Although multiple events occur in a particular order during spermiogenesis, it is unclear how the timing of those events is controlled. In the present study, we found that primary spermatocytes obtained from the spermatogenic testes of Oryzias latipes synchronously differentiated into sperm without contact with somatic cells in culture. Because those sperm can fertilize with mature eggs (Saiki et al., 1997), any events of spermiogenesis that are essential for achieving fertilization are completed in the in vitro spermiogenesis. In the in vitro spermiogenesis, the protamine gene expression was observed in the early period and mitochondrion localization was established in the same period. Those results suggest that both nuclear remodeling and organelle replacement begin in the early period of spermiogenesis. The cytoplasmic lobe was formed after the mitochondrion localization had been established. In most spermatids differentiated in cell culture, a flagellum began to elongate during the early period and continued to elongate up to 3 days. These results revealed the timings of the spermiogenetic events under the intrinsic control of the cultured spermatids toward the formation of fertilizable sperm in O. latipes. |
| Databáze: | OpenAIRE |
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