Cytological, molecular mechanisms and temperature stress regulating production of diploid male gametes in Dianthus caryophyllus L
| Autor: | Mo Xijun, Gui Min, Ying Luo, Yalian Jiang, Wenru Tang, Zhou Xuhong, Lulin Ma, Shi Ziming, Wu Xuewei |
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| Rok vydání: | 2015 |
| Předmět: |
Physiology
Plant Science Carnation Flowers Genes Plant Chromosomes Plant Meiosis Dianthus Gene Expression Regulation Plant Stress Physiological Genetics medicine RNA Messenger Cloning Molecular Gene Gametogenesis Phylogeny Plant evolution Male meiosis II biology Temperature food and beverages Gene Expression Regulation Developmental biology.organism_classification Diploidy medicine.anatomical_structure Organ Specificity Gamete Pollen Ploidy Germ Cells Plant |
| Zdroj: | Plant physiology and biochemistry : PPB. 97 |
| ISSN: | 1873-2690 |
| Popis: | In plant evolution, because of its key role in sexual polyploidization or whole genome duplication events, diploid gamete formation is considered as an important component in diversification and speciation. Environmental stress often triggers unreduced gamete production. However, the molecular, cellular mechanisms and adverse temperature regulating diplogamete production in carnation remain poorly understood. Here, we investigate the cytological basis for 2n male gamete formation and describe the isolation and characterization of the first gene, DcPS1 (Dianthus Caryophyllus Parallel Spindle 1). In addition, we analyze influence of temperature stress on diploid gamete formation and transcript levels of DcPS1. Cytological evidence indicated that 2n male gamete formation is attributable to abnormal spindle orientation at male meiosis II. DcPS1 protein is conserved throughout the plant kingdom and carries domains suggestive of a regulatory function. DcPS1 expression analysis show DcPS1 gene probably have a role in 2n pollen formation. Unreduced pollen formation in various cultivation was sensitive to high or low temperature which was probably regulated by the level of DcPS1 transcripts. In a broader perspective, these findings can have potential applications in fundamental polyploidization research and plant breeding programs. |
| Databáze: | OpenAIRE |
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