A label-free electrochemical immunosensor for hepatitis B based on hyaluronic acid-carbon nanotube hybrid film
| Autor: | Erika C.S. Lima, Diego G.A. Cabral, Rosa F. Dutra, Patrícia Moura |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Hepatitis B virus
Analytical chemistry 02 engineering and technology Carbon nanotube Biosensing Techniques Electrochemistry 01 natural sciences Analytical Chemistry law.invention Electron transfer Nanosensor law Limit of Detection Humans Hyaluronic Acid Detection limit Chemistry Nanotubes Carbon 010401 analytical chemistry virus diseases Electrochemical Techniques 021001 nanoscience & nanotechnology digestive system diseases 0104 chemical sciences Chemical engineering Linear range Electrode Surface modification 0210 nano-technology |
| Zdroj: | Talanta. 148 |
| ISSN: | 1873-3573 |
| Popis: | An electrochemical immunosensor developed for detection of antibodies to hepatitis B core protein (anti-HBc) is described. Anti-HBc is the earliest serological marker from hepatitis B virus (HBV) infection, remaining all life after contact with virus, being considered the most important marker for uses in screening of blood bank. A nanohybrid surface assembled onto a glassy carbon electrode consisting of amino carbon nanotubes recovered by hyaluronic acid was used as sensing platform to detect the anti-HBc. All the steps of electrode surface modification were characterized by Scanning Electronic Microscopy and extensively evaluated by electrochemical techniques. The electrode response was measured by direct anti-HBc antigen interactions by square wave voltammetry, dispensing uses of label or chemical mediators. Under optimal conditions, the anodic peak current which was proportional to the anti-HBs concentration. The immunosensor response was linear toward anti-HBc in concentrations up to 6 ng mL −1 , with a detection limit of 0.03 ng mL −1 . The linear range achieved was according to clinical level, indicating the immunosensor as promising tool for use as a criterion for blood bag disposal. The enhancement of the hyaluronic acid by carbon nanotube promoted an increase of charge electron transfer, besides a stable platform for HBc. |
| Databáze: | OpenAIRE |
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