Immunogold Electron Microscopic Localization of the 2 EF-Hand Calcium-Binding Pollen Allergen Phl p 7 and its Homologues in Pollens of Grasses, Weeds and Trees
| Autor: | Rudolf Valenta, Kerstin Westritschnig, Monika Grote |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Allergy
Pollen allergen Immunology chemistry.chemical_element Asteraceae Calcium Biology medicine.disease_cause Antibodies Trees Allergen immune system diseases Pollen otorhinolaryngologic diseases medicine Humans Immunology and Allergy Poaceae EF Hand Motifs Microscopy Immunoelectron EF hand Calcium-Binding Proteins food and beverages General Medicine Immunogold labelling Allergens Antigens Plant respiratory system medicine.disease Immunohistochemistry Protein Structure Tertiary respiratory tract diseases chemistry Structural Homology Protein Phleum Protein Binding |
| Zdroj: | International Archives of Allergy and Immunology. 146:113-121 |
| ISSN: | 1423-0097 1018-2438 |
| Popis: | Background: The 2 EF-hand calcium-binding allergen from timothy grass pollen, Phl p 7, contains the majority of relevant IgE epitopes among calcium-binding allergens occurring in pollen species of different plants. Objective: To describe the ultrastructural localization of Phl p 7 allergen in timothy grass pollen and its homologues in a broad spectrum of allergologically relevant pollens from grasses (timothy grass, rye grass), trees (birch, alder, olive) and weeds (mugwort, ribwort, ragweed) commonly growing in Europe. Materials and Methods: Mature pollens from 8 different plant species were collected and anhydrously prepared for transmission electron microscopy. In ultrathin sections, allergens were localized using an antibody prepared against a Phl p 7-derived peptide comprising the C-terminal half of the Phl p 7 wild-type molecule in combination with a secondary antibody coupled to 10-nm colloidal gold particles. Results: Phl p 7 and Phl p 7 homologues were detected in pollen from each of the 8 pollen species investigated. The allergens were found in the cytoplasm of the pollen grains (cytoplasmic matrix, mitochondria, nuclei) and in the pollen wall (preferably the exine). Reserve materials were unlabeled. Conclusions: The 2 EF-hand calcium-binding allergen Phl p 7 from timothy grass and its homologues can be localized in all pollen species under investigation. This finding confirms that Phl p 7 is a marker allergen for sensitization of patients to a novel family of 2 EF-hand calcium-binding pollen allergens occurring in a number of important allergenic plants in Europe. |
| Databáze: | OpenAIRE |
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