Effects of insulin-like growth factors on organotypic cocultures of embryonic rat brainstem slices and skeletal muscle fibres
| Autor: | I. Eustache, N. Seyfritz, J.P. Gueritaud |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Hypoglossal nucleus medicine.medical_treatment Muscle Fibers Skeletal Neuromuscular Junction Biology Embryonic and Fetal Development Insulin-like growth factor Organ Culture Techniques Developmental Neuroscience Insulin-Like Growth Factor II Tongue Neurotrophic factors Internal medicine medicine Animals Insulin-Like Growth Factor I Rats Wistar Muscle Skeletal Motor Neurons Skeletal muscle Embryonic stem cell Rats medicine.anatomical_structure Endocrinology Brainstem Neuroscience Brain Stem Developmental Biology Explant culture |
| Zdroj: | Developmental Brain Research. 81:284-292 |
| ISSN: | 0165-3806 |
| Popis: | Embryonic rat brainstem slices including the facial and hypoglossal motor nuclei were maintained in organotypic cocultures with explants of embryonic tongue or post-natal skeletal muscle for periods up to 3 weeks. Survival and differentiation of motoneurones were dependent both on the type of muscle explant and its position relative to the brainstem. Tongue explants induced a more important glial outgrowth, a motoneurone migration towards the muscle, earlier muscular contractions and a more complete neuronal and muscular differentiation. Since the foetal tongue contains IGF levels as high as foetal liver, these effects might be due in part to diffussion of IGF from the explanted muscle. Indeed, foetal liver explants or crude foetal liver extracts induced effects similar to those of tongue explants. These effects can be reproduced by addition of IGF-1 or IGF-2, or both, into the culture medium. Although IGF-1 and IGF-2 had similar effects, IGF-1 induced a more pronounced muscular differentiation and IGF-2 promoted neuronal differentiation. Our results suggest that IGFs are good candidates as muscle-derived neurotrophic factors promoting survival and differentiation of rat cranial motoneurones. These results also stress the importance of neuroglial trophic interactions and target development. |
| Databáze: | OpenAIRE |
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